ICAR - Central Soil Salinity Research Institute (CSSRI)

Principal Investigator (PI)

Dr. Anita Mann

Principal Scientist

Co-Principal Investigators (Co-PIs)

Dr. S.K. Sanwal

Principal Scientist

Dr. Neeraj Kulshreshtha

Principal Scientist

Dr. Arvind Kumar

Senior Scientist

Dr. Jogendra Singh

Senior Scientist

Dr. Ashwani Kumar

Senior Scientist

Ms. Avni Dahiya

Scientist

Dr. Vijayata Singh

Scientist

Dr. Lokesh B.M.

Scientist

Dr. Nitish Ranjan Prakash

Scientist

Target Traits in Crops

Research Methods & Expected Outcomes

Guide RNA designing has been successfully completed for all the targeted genes. The designed guide RNAs were cloned into the pYL-CRISPR-Ubip vector for construct development. In crop transformation, two cycles of transformation have been attempted for several target genes associated with key agronomic traits, while transformation has also been initiated for apomixis.