ICAR-Indian Institute of Soybean Research

Collaborating Centre Principal Investigator (CC-PI)

Dr. Milind B. Ratnaparkhe

Principal Scientist

Principal Investigators involved in the project

Dr. Milind B. Ratnaparkhe

Principal Scientist

Dr. Sanjay Gupta

Principal Scientist

Dr. Giriraj Kumawat

Senior Scientist

Co-Principal Investigators (Co-PIs)

Dr. Giriraj Kumawat

Senior Scientist

Dr. Sanjay Gupta

Principal Scientist

Dr. Shiva Kumar

Senior Scientist

Dr. Prince Choyal

Scientist

Dr. Lokesh Meena

Scientist

Target Traits in Soybean

Research Methods & Expected Outcomes

We have designed a total of twelve guide RNAs for the selected targets (two for each, including homologues). Out of these, six have been cloned into the pBlu entry vector and confirmed through PCR and sequencing. From the pBlu entry vector, the guide RNA cassette was further cloned into the MDC123 binary vector, which was also PCR confirmed.

For standardization of the plant transformation protocol, we are using the Cas9 MDC123 empty vector along with Agrobacterium (EHA105)-mediated transformation. Regeneration protocols in all selected genotypes have been tested and optimized.