ICAR - National Institute of Abiotic Stress Management

Collaborating Centre Principal Investigator (CC-PI)

Dr. Ajay K Singh

Principal Scientist

Principal Investigator involved in the project

Dr. Ajay K Singh

Principal Scientist

Co-Principal Investigators (Co-PIs)

Dr. Boraiah KM

Scientist

Dr. Basavaraj PS

Scientist

Dr. Pratapsingh Khapte

Scientist

Dr. Sushil Changan

Scientist

Dr. Aliza Pradhan

Scientist

Dr. GC Wakchaure

Senior Scientist

Dr. Sushma Awaji

Scientist

Dr. K Sammi Reddy

Director

Target Traits in Soybean

Research Methods & Expected Outcomes

Guide RNAs were designed for key regulatory and stress-response genes in soybean. Gene constructs were developed for knockout of these target genes. Agrobacterium-mediated genetic transformation was standardized in soybean, and putative genome-edited plants were successfully generated. An in-planta transformation method was also standardized to produce additional genome-edited lines. Furthermore, a virus-based system was developed for generating edited mutant lines, with guide RNAs inserted into the viral vector and the Cas9 cassette incorporated into the pCAMBIA1301 vector. Putative transformants carrying Cas9 were developed using Agrobacterium-mediated transformation.